Journal Articles



  1. Entcheva E. Uncovering an electrically heterogeneous cardiomyocyte by FRAP-quantified diffusion in the T-tubules. Proceedings of the National Academy of Sciences, doi: 10.1073/pnas.1719550115, 2018.
  2. Klimas A, Ambrosi CM, Yu J, Williams JC, Bien H, and Entcheva E. OptoDyCE as an Automated System for High-Throughput All-Optical Dynamic Cardiac Electrophysiology. Nature Communications, 7:11542. doi: 10.1038/ncomms11542, 2016. [PDF]
    Software can be found here.
    Media Coverage: Researchers use light to control human heart cells, expedite development of new drugs
  3. Entcheva E and Bub G. All-optical control of cardiac excitation: Combined high-resolution optogenetic actuation and optical mapping. Journal of Physiology, doi: 10.1113/JP271559. [Epub ahead of print] 2016 (invited perspective).
  4. Yu J and Entcheva E. Inscribing optical excitability to non-excitable cardiac cells: viral delivery of optogenetic tools in primary cardiac fibroblasts. Methods in Molecular Biology, doi: 10.1007/978-1-4939-3512-3_21, 1408:303-17, 2016. (Invited Article) [PDF]
  5. Ambrosi CM, Boyle PM, Chen K, Trayanova NA, and Entcheva E. Optogenetics-enabled assessment of viral gene and cell therapy for restoration of cardiac excitability. Scientific Reports, doi:10.1038/srep17350, 2015. [PDF]
  6. Burton RAB, Klimas A, Ambrosi CM, Tomek J, Corbett A, Entcheva E, and Bub G. Optical control of excitation waves in cardiac tissue. Nature Photonics, 9(12), 813-816, 2015 [PDF] (Media Coverage)
  7. Entcheva E. A step closer to cardiac optogenetics in vivo. (Invited Editorial), Cardiovascular Research, 106(2):180-1. doi: 10.1093/cvr/cvv116, 2015. [PDF]
  8. Boyle PM, Karathanos TV, Entcheva E, and Trayanova NA. Computational modeling of cardiac optogenetics: Methodology overview and review of findings from simulations. Computers in Biology and Medicine, doi:10.1016/j.compbiomed.2015.04.036. 2015.[PDF]
  9. Williams JC and Entcheva E. Optogenetic versus Electrical Stimulation of Human Cardiomyocytes: Modeling Insights. Biophysical Journal,doi:10.1016/j.bpj.2015.03.032. 2015. [PDF]
    Software can be found here.
  10. Entcheva E. A step closer to cardiac optogenetics in vivo. (Invited Editorial), Cardiovascular Research, 106 (2):180-1. doi: 10.1093/cvr/cvv116. 2015. [PDF]
  11. Klimas A and Entcheva E. Towards microendoscopy-inspired cardiac optogenetics in vivo: technical overview and perspective. Journal of Biomedical Optics, 19(8):080701. doi: 10.1117/1.JBO.19.8.080701, 2014. [PDF]
  12. Entcheva E and Williams JC. Channelrhodopsin2 current during the action potential: “Optical AP clamp” and approximation. Scientific Reports, doi:10.1038/srep05838, 2014. [PDF]
  13. Ambrosi CM, Klimas A, Yu JZ, and Entcheva E. Cardiac applications of optogenetics. Progress in Biophysics & Molecular Biology, doi: 10.1016/j.pbiomolbio.2014.07.001, 2014. [PDF]
  14. Ambrosi CM and Entcheva E. Optogenetic control of cardiomyocytes via viral delivery. Methods in Molecular Biology, 1181, doi: 10.1007/978-1-4939-1047-2_19, 2014. [PDF]
  15. Wu CY, Chen B, Jiang YP, Jia Z, Martin DW, Liu S, Entcheva E, Song LS, and Lin RZ. Calpain-dependent cleavage of junctophilin-2 and T-tubule remodeling in a mouse model of reversible heart failure, Journal of the American Heart Association, PMID: 24958777, 3(3):e000527, 2014. [PDF]
  16. Entcheva E. Fiat lux in understanding cardiac pacing, resynchronization and signaling by way of optogenetics, (Invited Editorial), Cardiovascular Research, PMID: 24760549, 102(3):342-3, 2014. [PDF]
  17. Ambrosi, C. M., and E. Entcheva. Optogenetics’ promise: pacing and cardioversion by light? Future Cardiol., 2014 Jan;10(1):1-4. [PDF]
  18. Boyle, P. M., E. Entcheva, and N. A. Trayanova. See the light: can optogenetics restore healthy heartbeats? And, if it can, is it really worth the effort? Expert Rev Cardiovasc Ther., 2014 12(1): 17-20. [PDF]
  19. Williams JC, Xu J, Lu Z, Klimas A, Chen X, Ambrosi CM, Cohen IS, and Entcheva E,
    Computational Optogenetics: Empirically-Derived Voltage- and Light-Sensitive Channelrhodopsin-2 Model,
    PLoS Computational Biology, 2013 Sep;9(9):e1003220.
    (Open Access)Software Downloads:
    Code 1: ChR2 Model Only
    Code 2: ChR2 in Human Ventricular Myocyte Cell
  20. Boyle PM, Williams JC, Ambrosi CM, Entcheva E, Trayanova NA, A comprehensive multiscale framework for simulating optogenetics in the heart, Nature Communications, 2013 Aug 28;4:2370. [PDF;Supplementary Information]
  21. Entcheva E, Cardiac Optogenetics. Am J Physiol Heart Circ Physiol., 2013 May;304(9):H1179-91. [PDF]
  22. Entcheva E, Integration of optical stimulation with high-resolution optical imaging of cardiac tissue, Microscopy & Analysis, 27(1): D4, 2013.
  23. Lu J, Wang HZ, Jia Z, Zuckerman J, Lu Z, Guo Y, Boink GJ, Brink PR, Robinson RB, Entcheva E, Cohen IS., Improving cardiac conduction with a skeletal muscle sodium channel by gene and cell therapy, J Cardiovasc Pharmacol., 60(1):88-99, July 2012.
  24. Boink GJ, Lu J, Driessen HE, Duan L, Sosunov EA, Anyukhovsky EP, Shlapakova IN, Lau DH, Rosen TS, Danilo P, Jia Z, Ozgen N, Bobkov Y, Guo Y, Brink PR, Kryukova Y, Robinson RB, Entcheva E, Cohen IS, and Rosen MR. Effect of SkM1 sodium channels delivered via a cell platform on cardiac conduction and arrhythmia induction, Circ Arrhythm Electrophysiol., 21 June 2012.
  25. Jia Z, Bien H, Shiferaw Y and Entcheva E, Cardiac cellular coupling and the spread of early instabilities in intracellular Ca2+, Biophysical Journal, 102(6):1294-302, 2012. [PDF]
  26. Jia Z, Valiunas V, Lu Z, Bien H, Wang HZ, Liu H, Rosati B, Brink, PR, Cohen IS and Entcheva E, Stimulating cardiac muscle by light: cardiac optogenetics by cell delivery,Circulation: Arrhythmia & Electrophysiology, 4(5):753-60, 2011. [PDF]
  27. Wu, CY, Jia Z, Wang W, Ballou LM, Jiang YP, Chen B, Mathias RT, Cohen IS, Song LS, Entcheva E, and Lin RZ, PI3Ks maintain the structural integrity of T-tubules in cardiac myocytes, PLoS One, 6(9):e24404, 2011.
  28. Quinn TA,…..,Entcheva E,….. Kohl P, Minimum information about a cardiac electrophysiology experiment (MICEE): Standardised reporting for model reproducibility, interoperability, and data sharing, Prog Biophys Mol Biol, 107(1):4-10, 2011.
  29. Leigh S, Tattu A, Mitchell JSB and Entcheva E, M3: Microscope-based Maskless Micropatterning with Dry-Film Photoresist, Biomedical Microdevices, 13(2):375-81, 2011.
  30. Chung CY, Bien H, Sobie EA, Dasari V, McKinnon D, Rosati B and Entcheva E, Hypetrophic phenotype in cardiac cell assemblies solely by structural cues and ensuing self-organization, FASEB Journal, 25(3):851-62, 2011.
  31. Horning M, Isomura A, Jia Z, Entcheva E, Yoshikawa K, Utilizing the eikonal relationship in strategies for reentrant wave termination in excitable media. Phys Rev E, 81(5): 056202, 2010.
  32. Shah U, Bien H, and Entcheva E, Microtopographical effects of natural scaffolding on cardiomyocyte function and arrhythmogenesis. Acta Biomaterialia, 6(8):3029-34, 2010.
  33. Wang W, Gao J, Entcheva E, Cohen IS, Mathias RT, A transmural gradient in the cardiac Na/K pump generates a transmural gradient in Na/Ca exchange. J Membrane Biology, 233(1-3):51-62, 2010.
  34. Jia Z, Bien H, and Entcheva E, Detecting space-time alternating biological signals close to the bifurcation point. IEEE Trans Biomedical Eng, 57(2):316-24, 2010.
  35. Lu Z, Jiang YP, Wang W, Xu XH, Mathias RT, Entcheva E, Ballou LM, Cohen IS and Lin RZ, Loss of cardiac phosphoinositide 3-kinase p110a results in contractile dysfunction, Circulation , 120(4):318-25, 2009.
  36. Bartocci E, Corradini F, Di Berardini MR, Entcheva E, Smolka SA, and Grosu, R, Modeling and simulation of cardiac tissue using hybrid I/O automata, Theoretical Computer Science, 410(33-34):3149-3165, 2009.
  37. Grosu R, Smolka SA, Corradini F, Wasilewska A, Entcheva E, Bartocci E, Learning and detecting emergent behavior in networks of cardiac myocytes, Communications of the ACM, 52(3):97-105,  2009.
  38. Entcheva E and Bien H, Mechanical and spatial determinants of cytoskeletal geodesic dome formation in cardiac fibroblasts. Integrative Biology, 1(2):212-219, 2009.
  39. Protas L, Dun W, Jia Z, Lu J, Bucchi A, Kumari S, Lu Z, Chen M, Cohen IS, Rosen MR, Entcheva E, and Robinson RB, Expression of skeletal but not cardiac Na+ channel isoform preserves normal conduction in a depolarized cardiac syncytium, Cardiovascular Research, 81(3):528-535, 2009.
  40. Grosu R, Bartocci E, Corradini F, Entcheva E, Smolka SA, Wasilewska A, Learning and detecting emergent behavior in networks of cardiac myocytes, LNCS: Lecture Notes in Computer Science, 4981:229-243, 2008.
  41. Bartocci E, Corradini F, Entcheva E, Grosu, R, Smolka SA, CellExcite: A tool for simulating excitable cells, BMC Bioinformatics, 9, Suppl 2:S3, 2008.
  42. Ye P, Grosu R, Smolka SA, and Entcheva E, Formal analysis of abnormal excitation in cardiac tissue, LNBI: Lecture Notes in Bioinformatics, 5307:141-155, 2008.
  43. Bartocci E, Corradini F, Di Berardini MR, Entcheva E, Grosu R, Smolka SA. Spatial I/O hybrid automata for excitable tissue modelling. ENTCS: Electronic Notes in Theoretical Computer Science, 194:51-67, 2008.
  44. Ye P, Entcheva E, Smolka SA, and Grosu R. Modeling excitable cells using cycle-linear hybrid automata. IET Systems Biology 2:24-32, 2008.
  45. Chung CY, Bien H and Entcheva E. The role of cardiac tissue alignment in modulating electrical function. Journal of Cardiovascular Electrophysiology 18:1323-1329, 2007.
  46. Grosu R, Mitra S, Ye P, Entcheva E, Ramakrishnan IV, and Smolka SA. Learning cycle-linear hybrid automata for excitable cells. Lecture Notes in Computer Science 4416 , 245-258, 2007.
  47. Bien H, Parikh P, and Entcheva E. Lenses and effective spatial resolution in macroscopic optical mapping. Physics in Medicine and Biology 52(4):941-960, 2007.
  48. Bien H and Entcheva E. Spatial frequency content in optical mapping of cardiac cell monolayers. American Journal of Physiology – Heart and Circ Physiol 291(3):H484-5, 2006.
  49. Bien H, Yin L, and Entcheva E. Calcium instabilities in mammalian cardiomyocyte networks. Biophysical Journal 90: 1-13, April 2006.
  50. Entcheva E and Bien H. Macroscopic optical mapping of excitation in cardiac cell networks with ultra-high spatiotemporal resolution. Progress in Biophysics and Molecular Biology 92(2):232-57, 2006. featured in the December 2006 issue of Biophotonics International
  51. Ye P, Entcheva E, Grosu R, and Smolka SA. Efficient modeling of excitable cells using hybrid automata. Computational Methods in Systems Biology. 2005.
  52. Zong X, Bien H, Chung CY, Yin L, Fang D, Hsiao BS, Chu B, and Entcheva E. Electrospun fine-textured scaffolds for heart tissue constructs. Biomaterials. 26: 5330-8, 2005.
  53. Entcheva E and Bien H. Acoustic micromachining of three-dimensional surfaces for biological applications. Lab on a Chip 2005. 5(2): 179-184. “hot article” according to the Royal Society of Chemistry
  54. Yin L*, Bien H*, Entcheva E. Scaffold topography alters intracellular calcium dynamics in cultured cardiomyocyte networks. American Journal of Physiology – Heart & Circulatory Physiology. 283(3): H1276-85, 2004. (*equal contribution)
  55. Entcheva E, Bien H, Yin L, Chung C-Y, Farrell M, and Kostov Y. Functional cardiac cell constructs on cellulose-based scaffolding. Biomaterials. 25: 5753-5762, 2004.
  56. Entcheva E, Kostov Y, Tchernev E, and Tung L. Fluorescence imaging of electrical activity in cardiac cells using an all-solid-state system. IEEE Trans Biomed Eng . 51(2): 333-341, 2004.
  57. Bien H, Yin L and Entcheva E. Cardiac cell networks on elastic microgrooved scaffolds. IEEE Eng Med Biol. 22(5): 108-112, 2003.
  58. Entcheva E and Bien H. Tension development and nuclear eccentricity in topographically controlled cardiac syncytium. J Biomedical Microdevices. 5(2): 165-170, 2003.